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bodipy cholesterol invitrogen

Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. Two-photon time-lapse microscopy of BODIPY-cholesterol reveals ... Many BODIPY deriv-atives, including labeled fatty acids and complex lipid probes, have been synthesized (12, 13) and applied in nu-merous biological studies (1, 14). KRAS (G12C), BODIPY-GDP Loaded, His-tag - BPS Bioscience Specifications Product Type Cholesterol Analogs Excitation/Emission 505/511 nm Product Line BODIPY™ Contents & Storage Synthesis of Cholesterol Analogues Bearing BODIPY ... - DeepDyve TMEM41B and VMP1 are scramblases and regulate the distribution of ... BODIPY® 492/515 Life Technologies D3922 0.1M Phosphate Buffered Saline pH 7.4 coverslip Corning 2935-245 Protocol: WARNING: Paraformaldehyde is, toxic, flammable and considered a carcinogen Xylene, ethanol and methanol are all flammable and should be used in fume hood away from open flames or sparks . Di-4 ANEPPDHQ (Invitrogen) in dimethyl sulfoxide (DMSO) was added to a concentration of 3 µg/ml, and erythrocytes at ≈0.1 hematocrit were incubated for 30 min at RT. For the BODIPY-cholesterol assay, ram spermatozoa were labelled with BODIPY-cholesterol and prepared for in vitro capacitation as previously described (Bernecic et al. Y3603) from Molecular Probes . retinoic acid, cholesterol, actinomycin D and gemcit-abine hydrochloride (USP standard) (Sigma); insulin (ermoFisher); protein A/G agarose beads (Millipore); BODIPY dyes (ermoFisher). Because of its unique spectral properties and superior photo-stability compared to other fluorophores such as nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) and fluorescein, we subsequently generated other Bodipy-analogs for lipid trafficking studies. neurons indeed influence the trafficking behavior of BODIPY- lac-Cer. PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol ... M24571) and Yo-Pro-1 (catalogue no. . Structures of Bodipy-cholesterol, Bodipy-cholesteryl oleate, and ...

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